Effect of Artemisia frigida Total Flavonoids on the Levels of LTB4,5-HETE,Ca2+ and Cyclic AMP in Rat Peritoneal Leukocytes

doi:10.16333/j.1001-6880.2016.4.022

Abstract

Abstract: In this study,rat neutrophils from pleural fluid was used as experimental material to determine the levels of LTB4,5-HETE,Ca²⁺ and cAMP with HPLC,RIA and Fura-2/ AM,respectively.As a results, Artemisia frigida total flavonoids inhibited the formation of LTB4 from the intact neutrophils with its concentration increasing from 2.0 to 10.0 mg/L,and inhibited the Ca²⁺ stimulated by fMLP and promoted the improvement of cAMP in the cells.The effects of A.frigida total flavonoids on leukocyte function may be the mechanisms of its antiinflammatory action.

Key words: Artemisia frigida, total flavonoid, LTB4, Ca²⁺, cAMP

CLC Number:

R914

HUA La,WANG Qing-hu*,DAI Na-yin-tai,NA Ren-chao-ke-tu,WU Rong-jun,WU Jie-si. Effect of Artemisia frigida Total Flavonoids on the Levels of LTB4,5-HETE,Ca²⁺ and Cyclic AMP in Rat Peritoneal Leukocytes[J]. NATURAL PRODUCT RESEARCH AND DEVELOPMENT, 2016, 28(4): 591-595.

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Effect of Artemisia frigida Total Flavonoids on the Levels of LTB4,5-HETE,Ca²⁺ and Cyclic AMP in Rat Peritoneal Leukocytes

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