Abstract: Eight compounds including two new compounds,namely shushenes A (1) and B (2),were isolated from the fruiting bodies of Ganoderma applanatum. Their structures were identified by means of spectroscopic methods.The structures of the known compounds are lucidulactone A (3),2,3-dihydroxy-1-(4-hydroxy3,5-dimethoxyphenyl)-1-propanone (4),6-hydroxy-4-H-chromen-4-one (5),1-(4-ethylphenyl)-1,2-ethanediol (6),1-(2-ethylphenyl)-1,2-ethanediol (7) and indole-3-carboxaldehyde (8),which were all isolated from this species for the first time.Of note,compounds 1 and 2 were isolated as racemic mixtures,chiral separation followed by computational methods allowed their absolute configuration assignment.In addition,inhibition of compounds 1 and 2 on fibronectin expression was tested by using an enzyme-linked immunosorbent assay (ELISA).