Screening and Identification of Strains Transforming Baccatin III into 10-Deacetyl Baccatin III

doi:10.16333/j.1001-6880.2016.7.024

NATURAL PRODUCT RESEARCH AND DEVELOPMENT ›› 2016, Vol. 28 ›› Issue (7): 1128-1134.doi: 10.16333/j.1001-6880.2016.7.024

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Screening and Identification of Strains Transforming Baccatin III into 10-Deacetyl Baccatin III

ZHU Feng-zhi,YANG Ya-nan,ZHANG Kun,ZHANG Ao-wei,WANG Min,LUO Jian-mei*

Key Laboratory of Industrial Fermentation Microbiology(Tianjin University of Science &Technology),Ministry of Education; Tianjin Key Lab of Industrial Microbiology,College of Biotechnology,Tianjin University of Science and Technology,Tianjin 300457,China

Online:2016-07-30 Published:2017-01-17

Abstract

Abstract: 10-Deacetyl baccatin III(10-DAB)is the synthetic precursor of the taxanes drug docetaxel with anti-tumor activity.By the biotransformation of baccatin III,six 10-DAB producing strains isolated from the sludge were screened by the combinational methods of TLC,HPLC and LC-MS,which were named as P1-A-19,P2-A-8,P2-A-10,P2-A-11,P2-A-12 and P2-A-13,respectively.Combining with morphological observation,physiological and biochemical characteristics,16S rDNA sequence analysis,P1-A-19 was identified as Raoultella ornithinolytica, P2-A-8 was identified as Pantoea agglomerans, P2-A-10,P2-A-11,P2-A-12,P2-A-13 were identified as Pseudomonas aeruginosa. Raoultella ornithinolytica P1-A-19 was firstly detected to produce 10-DAB from baccatin III.

Key words: 10-DAB, baccatin III, biotransformation, docetaxel, strain identification

CLC Number:

Q939.9

ZHU Feng-zhi,YANG Ya-nan,ZHANG Kun,ZHANG Ao-wei,WANG Min,LUO Jian-mei*. Screening and Identification of Strains Transforming Baccatin III into 10-Deacetyl Baccatin III[J]. NATURAL PRODUCT RESEARCH AND DEVELOPMENT, 2016, 28(7): 1128-1134.

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Screening and Identification of Strains Transforming Baccatin III into 10-Deacetyl Baccatin III

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