NATURAL PRODUCT RESEARCH AND DEVELOPMENT ›› 2016, Vol. 28 ›› Issue (7): 1139-1143. doi: 10.16333/j.1001-6880.2016.7.026

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Effect of Water Extract of Shizidaiping Prescription on Insulin Resistance of Skeletal Muscle Cells

ZHOU Xue-mei1,TIAN Chun-yu1,LA Xiao-jin1,CHEN Guo-lin2,ZHANG Bi-wei1,FU Qian-ru1,CHENG Rui-ting1,LI Ji-an1*   

  1. 1 College of Traditional Chinese Medicine,North China University of Science and Technology,Tangshan 063000,China; 2 Hebei Wen’an County Hospital,Wen’an 065800,China
  • Online:2016-07-30 Published:2017-01-17

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Abstract: To observe the effect of Shizidaiping formula on insulin resistance skeletal muscle cells and to explore its mechanism in improving insulin resistance.Insulin resistant model was developed with 5×107mol/L insulin intervention for 12 h.High,middle and low concentrations of Shizidaiping formula(400,100,25 μg/mL)(SZDP-H,SZDP-M and SZDP-L)were then applied on skeletal muscle cells.Pioglitazone(40 μmol/L)was used as positive control.The content of glucose in skeletal muscle cell supernatant was determined by glucose oxidase method and the expressions of AKT and GSK-3β was detected with Western-blot method.The results showed that Shizidaiping formula can improve glucose metabolism,increase the content of AKT and AKT phosphorylation level,decrease the content of GSK-3β,and increase GSK-3β phosphorylation level.Shizidaiping formula improve glucose metabolism through increasing phosphorylation levels of AKT and GSK-3β.

Key words: Shizidaiping formula, skeletal muscle cell, insulin resistance, AKT/GSK-3&beta

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