NATURAL PRODUCT RESEARCH AND DEVELOPMENT ›› 2016, Vol. 28 ›› Issue (7): 991-995.doi: 10.16333/j.1001-6880.2016.7.001

• Article •     Next Articles

Isolation and Preliminary Toxicity Evaluation of Alkaloids from Stephania dicentrini fera H.S.Lo & M.Yang

YAN Fei1,2,MA Yun-shu1*,WANG Bo1,CHEN Cheng1,WANG Hui1   

  1. 1 College of Pharmacy,Yunnan University of Traditional Chinese Medicine,Kunming 650500,China; 2 College of Pharmacy,Shaanxi University of Chinese Medicine,Xi’an 712046,China
  • Online:2016-07-30 Published:2017-01-17

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Abstract: In this study,aporphine(AP)-type alkaloids were isolated from Stephania dicentrinifera H.S.Lo & M.Yang by silica gel column chromatography.Their structures were identified by TLC,UV,NMR,MS, etc.The acute toxicity of 3 obtained alkaloids ［Dicentrinone(Dico)and Dicentrine(Dic)of AP-type alkaloids, Tetrahydropalmatine(Tet) of Protoberberine(PBB)-type alkaloids］ was then investigated using the method of tail vein injection in mice,their LD50's were calculated by Bliss method.The experimental results showed that the LD50 values of Dico,Dic and Isocorydine(Isoc)were 7.266 mg/kg,26.802 mg/kg and 32.167 mg/kg,respectively.Dico was separated from S.dicentrinifera for the first time,and its acute toxicity was higher than Dic and Isoc.

Key words: Stephania.dicentrinifera H.S.Lo &, M.Yang;aporphine alkaloids;dicentrinone;dicentrine;isocorydine;LD50

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