NATURAL PRODUCT RESEARCH AND DEVELOPMENT ›› 2016, Vol. 28 ›› Issue (8): 1238-1243.doi: 10.16333/j.1001-6880.2016.8.012

• Article • Previous Articles     Next Articles

Determination of Main Components in Bulbs of Wild Lycoris sprengeri from Ningbo

DAI Zhi-Hui1,LONG Jun2,3,YU Lei-min1,4,NI Sui1*   

  1. 1Ningbo University,Ningbo 315211,China;2Nanjing Forestry University,Nanjing 210037,China;3Garden Management  Office of Ningbo City,Ningbo 315010,China;4Xiangshan Forestry Specialty Technology Promotion Center,Xiangshan 315700,China
  • Online:2016-08-30 Published:2017-01-17

Abstract: To determine the content of main components in bulbs of wild Lycoris sprengeri from different places of Ningbo.The bulbs of wild L.sprengeri collected from Zhoushan,Beilun,Fenghua,Xiangshan,Zhenhai,Cixi were used as experimental material.The content of lycorine was equal to the content of total alkaloid.The content of total flavones,nonstarch polysaccharides and total alkaloids were analyzed by spectrophotometer and the content of galanthamine was determined using HPLC.The results indicated that there existed significant differences in the content of main components in bulbs of wild L.sprengeri from different places.The content of total flavones,total alkaloids,galanthamine and nonstarch polysaccharides of the bulbs of wild L.sprengeri were from 1.095 to 1.751 mg/g,2.691 to 5.559 mg/g,0.686 to 1.476 mg/g and 11.590% to 14.182% respectively.The samples from Hepu of Xiangshan had the highest content of total flavones,which was (1.751±0.116) mg/g.The samples from Haihuang mountain of Cixi had the highest content of nonstarch polysaccharides,which was (14.182±0.523)%.The samples from Zhaobao mountain of Zhenhai had the highest content of total alkaloids,which was (5.559±0.394) mg/g.The samples from Yangzhangao of Beilun had the highest content of galanthamine,which was (1.476±0.018) mg/g.Correlation analysis showed that the relationship between the contents of total alkaloids and galanthamine was positive in the same place,the correlation coefficient was 0.601.However it was not significant.These results provided reference for the optimization and development in the artificial cultivation of L.sprengeri.

Key words: wild Lycoris sprengeri, Ningbo area, main component, galanthamine, content determination

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