NATURAL PRODUCT RESEARCH AND DEVELOPMENT ›› 2016, Vol. 28 ›› Issue (8): 1262-1265.doi: 10.16333/j.1001-6880.2016.8.016

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Purification and Activity Determination of Polysaccharides from Maojian Tea

QU Wei1,LIU Zhuang1,ZHANG Zhao-kang2,CHENG Xiang-yi2,FAN Hai-tao3,4*   

  1. 1 Hospital of Beijing Union University,Beijing 100101,China;2 Department of Physics and Institute of Biophysics, Central China Normal University,Wuhan 100191,China;3 College of Bioengineering,Beijing Polytechnic,Beijing 100176,China;4 Institute of Pharmacology and Toxicology,Academy of Military Medical Sciences,Beijing 100850,China
  • Online:2016-08-30 Published:2017-01-17

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Abstract: To investigate the structure and activity of polysaccharides (MTP) from Maojian Tea.The polysaccharides were extracted and purified from Maojian Tea to afford an uniform polysaccharide,namely MTP.The effect of MTP on DPPH· scavenging assay,cell viability,phagocytosis capability and NO production of RAW264.7 macrophages were investigated.The results showed that viability,phagocytosis capability and NO production of RAW264.7 macrophages had been stimulated by MTP at the concentration of 500 μg/mL.The presented results confirmed MTP had capacity of eliminating DPPH radicals and cellstimulating activity.

Key words: Maojian Tea, polysaccharides, activity, macrophages

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