Abstract: In this study,eucommiol was isolated and purified from leaves of Eucommia ulmoides Oliv.by liquid-liquid extraction,macroporous resin adsorption and semi-preparative HPLC.Firstly,the crude extract was extracted by ultrasonic-assisted extraction using n-butanol as extraction solvent.Secondly,the n-butanol extract was separated by D101 macroporous resin column.Finally,eucommiol was prepared by reverse phase semi-preparative HPLC.The mobile phase of semi-preparative HPLC was methanol-water(V:V=15:85).The structure of the isolated eucommiol was subsequently confirmed by ultraviolet spectrophotometry(UV),infrared absorption spectroscopy(IR),nuclear magnetic resonance spectroscopy(NMR) and liquid chromatography-mass spectrometry(LC-MS).HPLC analyses were performed to test and verify the purity of eucommiol(95.12%).

Key words: Eucommia ulmoides Oliv.leaves, eucommiol, preparation, structural characterization