NATURAL PRODUCT RESEARCH AND DEVELOPMENT ›› 2016, Vol. 28 ›› Issue (Suppl1): 26-29.doi: 10.16333/j.1001-6880.2016.S.008

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Preparation and αAmylase Inhibition Activity of Tiliroside

ZHANG Yu1,MA Yanyan1，2,ZHAO Denggao1，2,ZHOU Aiyu2,DU Zhiyun1,ZHANG Kun1,2*   

  1. 1School of Chemistry & Environmental Engineering,Wuyi University,Jiangmen 529020,China; 2Institute of Natural Medicinal Chemistry & Green Chemistry,Department of Pharmaceutical Engineering, School of Light Industry and Chemical Engineering,Guangdong University of Technology,Guangzhou 510006,China
  • Online:2016-06-30 Published:2017-03-02

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Abstract: The aim of the research was to study the inhibition of tiliroside to αamylase，which was isolated from the flowers of Edgeworthia gardneri.Tiliroside was evaluated for its inhibitory activity against αamylase.The mode of inhibition for αamylase and oral sucrose tolerance test was also investigated.Tiliroside showed significant αamylase inhibitory activity (IC50 = 12.1 μM) in a noncompetitive fashion with a Ki of 9.72 μM.The results obtained from oral sucrose tolerance test revealed that tiliroside is able to inhibit αamylase at the intestinal level with consequent decrease of the postprandial peak in mice.This is the first report on the αamylase inhibition of tiliroside. In vitro and in vivo experiments showed that the inhibition of α amylase is one of the mechanisms of antihyperglycemic activity of E.gardneri.

Key words: tiliroside, Edgeworthia gardneri, &alpha, amylase, noncompetitive inhibition mode, oral sucrose tolerance test

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