NATURAL PRODUCT RESEARCH AND DEVELOPMENT ›› 2016, Vol. 28 ›› Issue (增刊2): 192-201.doi: 10.16333/j.10016880.2016.S.004

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Comparison on the Extraction and Determination Method of Total Flavonoids in Quinoa Seed

FENG Huanqin1,2,XU Xuefeng1,2,YANG Hongwei1,2,SHEN Baoyun3,HU Jing3,LI Chaozhou1,2*   

  1. 1Gansu Key Laboratory of Crop Genetics & Germplasm Enhancement; 2College of Life Science and Technology,Gansu Agricultural University,Lanzhou 730070,China; 3Agricultural and Forestal Science Research Institution of Gansu Tiaoshan Group,Lanzhou 730400,China
  • Online:2016-12-31 Published:2017-03-03

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Abstract: AlCl3 colorimetry method and Al(NO3)3 colorimetry method of total flavonoids from quinoa were studied by response surface methodology,in order to obtain the best extraction method.Based on single factor experiments,take ethanol concentration,extraction temperature and extraction time as main variables.With the total flavonoids yield,the content determination was optimized by response surface analysis method.The best extraction process for AlCl3 colorimetry method was to extract 1.1h in 62.45 ℃ water bath and 71.97% ethanol concentration,the yield of flavonoids was 5322% while error was 7.68%.The best extraction process for Al(NO3)3 colorimetry method was to extract 1.24 h in 61.13 ℃ water bath and 84.43% ethanol concentration,the yield of flavonoids was 8.799% while error was 1.24%.Compared with AlCl3 colorimetry method,Al(NO3)3 colorimetry method was efficient and stable for the determination of flavone in quinoa.

Key words: Response surface method,  Quinoa, AlCl3 colorimetry method, Al(NO3)3 colorimetry method

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