Abstract: Total alkaloids in Hypecoum leptocarpum Hook.f.et Thoms.were obtained from the ethanol extract using MCI gel.The antiinflammatory and analgesic effects of total alkaloids were studied using carrageenan induced edema and formalin test.The TLC identification of total alkaloids was carried out using GF254 silica gel plate with developing solvent of chloroformmethanol (6:1.5).The content of total alkaloids were determined by RPHPLC taking protopine as the index component.The results showed that total alkaloids in H.leptocarpum have edema inhibition and analgesic effect.The content of protopine in the total alkaloids was up to 13.3%.The calibration curve was in the range of 0.96-4.8 μg and the equation of regression was A = 6.37 × 106 X + 6.24×105，r=0.9999.The HPLC TLC method were accurate and simple.They can be used for the quality control of this active fraction in H.leptocarpum.

Key words: Hypecoum leptocarpum Hook.f.et Thoms., total alkaloids, antiinflammatory and analgesia, quality control