Abstract: To investigate the anti-inflammatory activity of Inula cappa extract from different solvents.Firstly,several parts with different polarity were acquired by enriching the compounds with D₁₀₁ macro-porous resin.Then,cell inflammatory model and anti-inflammatory model of animal were established to find out the most effective anti-inflammatory part of I.cappa extract.After the D₁₀₁ macroporous resin were washed using solvent with different polarities,five active constituents were got,including Fr.A,Fr.B,Fr.C,Fr.D and Fr.E.In addition,the washing part of 60% ethanol (Fr.E) showed a better anti-inflammatory effect comparing with others,whose activity of inhibiting the release of TNF-α and NO were 53.32±1.14,56.46±2.02,respectively.Furthermore,the inhibition of swelling rate of ear edema model of mouse was 54.81%.Therefore,the washing part of 60% ethanol (Fr.E) of I.cappa extract had the best anti-inflammatory effect.

Key words: Inula cappa, anti-inflammatory, nitric oxide, tumor necrosis factor