Abstract: The objective of study was to investigate the effect of compound malt pill(CMP) on the expression of LH and FSH in polycystic ovary syndrome model rats.The 9day-old rats were given the subcutaneous injection of testosterone propionate for inducing PCOS.The model rats were randomly divided into 5 groups(n=9): the model group,Diane 35 group,high,middle,low-dose CMP group.10 rats with normal estrus cycles were set as the normal group.The treatment groups were given intragastric administration with corresponding drugs,the normal group and the model group were given distilled water.The protein and mRNA expression levels of LH and FSH were detected by imunohistochemistry and PCR.The results showed that the protein and mRNA expression of LH were significantly increased in model group than that in normal group (P<0.01),compared with model group,the protein and mRNA expression of LH were significantly decreased in CMP each dose group (P<0.01),the protein expression of LH was significantly decreased in Diane-35 group (P<0.01) and the mRNA expression of LH was not significantly different(P>0.05).The protein and mRNA expression of FSH were significantly decreased in model group than that in normal group (P<0.01),the protein and mRNA expression of FSH were significantly increased in each treatment group than that in model group (P<0.01).Hence,it was concluded that,the protein and mRNA expression of LH was significantly increased and FSH was significantly decreased in PCOS model rats.The therapeutic effect of compound malt Pill on PCOS can be achieved,and its possible mechanism is related to regulating the protein and mRNA expression level of LH and FSH in ovarian tissue.

Key words: compound malt pill, polycystic ovary syndrome, LH, FSH