Abstract: In this study,the anti-inflammatory effect of major components of Forsythia suspensa was explored and its bioactive components with anti-inflammatory effect were screened to provide reference for the development of new anti-inflammatory drugs of F.suspensa. Then xylene-induced mice ear swelling models were established and the swelling degree was calculated.The content of tumor necrosis factor-α(TNF-α) and interleukin-6(IL-6) in mice serum were detected by enzyme-linked immunosorbent assay(ELISA).The swelling degree and the content of TNF-α and IL-6 in mice serum of medication administration groups were compared with those of control group respectively.Compared with the control group,phillyrin had no significant inhibition effect on swelling degree or the production of TNF-α and IL-6,and there was no significant difference between the two groups(P>0.05).Compared with the control group,significant differences of the swelling degree and the production of TNF-α and IL-6 were detected in phillygenin,forsythiaside A and forsythiaside B group respectively(P<0.05).Thus it can be seen that by oral administration,only the three selected components of F.suspense,including phillygenin,forsythiaside A and forsythiaside B,had anti-inflammatory effects.By oral administration,the anti-inflammatory effect of phillyrin had not been found yet,but phillygenin,forsythiaside A and forsythiaside B had stronger anti-inflammatory effects.Meanwhile,it can be speculated that the anti-inflammatory mechanism of F.suspensa may be related with its inhibition effect of the production of TNF-α and IL-6 and the effect of multiple components.

Key words: phillyrin, phillygenin, forsythiaside A, forsythiaside B, anti-inflammatory effect