Abstract: Two new compounds along with 21 known compounds were isolated from Tylotus bivittateinus. Their structures were identified as,(1R,4S,9S)-1-ethoxyoctahydro-5H,10H-dipyrrolo［1,2α:1,2′d］-pyrazine-5,10-dione(1),threo N-((4E)-1,3-dihydroxytetradec-4-en-2-yl)acetamide(2),3-［(3-phenyl-2-E-propen-1-yl)oxy］-1,2-propanediol(3),3-(2-hydroxyphenyl)-1-propanol(4),1-(2-ethylphenyl)-1,2-ethanediol(5),2-(2-hydroxyethyl)benzoic acid(6),1-phenyl-1,2,3propanetriol(7),1-phenyl-1,2-propanediol(8),1-phenyl-1-hydroxy acetic acid(9),3-hydroxy-3-(4-hydroxyphenyl)propanoic acid(10),methyl 3-phenylpropanoate(11),methyl 3-(4-hydroxyphenyl)propanoate(12),ethyl 3-(4-hydroxyphenyl)propanoate(13),4-hydroxybenzoic acid ethyl ester(14),benzoic acid ester(15),(E)-4methoxycinnamic acid(16),4-methoxyphenol(17),4-hydroxybenzaldehyde(18),4-methylphenol(19),4-methylacetophenone(20),benzoic acid(21),2hydroxybenzoic acid(22) and 3-hydroxybenzoic acid(23),respectively,by spectroscopic methods. The absolute configuration of 1 was assigned by ECD calculations. In addition,compounds 3-23 were isolated from T. bivittateinus for the first time.