Abstract: To investigate the effects and possible mechanism of aerobic exercise and spirulina supplement on the protein expression of TLR4/NF-κB-p65 as a target of improving renal injury and inflammatory factor in kidney tissue of type 2 diabetic rats. The model of type 2 diabetic rats was established through SD rats fed high-fat diet for four weeks together with intraperitoneal infecting a low dose of STZ. The model rats were randomly divided into diabetic control group(DM) and diabetic exercise group(DE) with intervention of swimming training for eight weeks,and diabetic plus spirulina group(DS) and diabetic exercise plus spirulina group(DES),additionally provided normal control group(NC) without any intervention. The blood glucose concentrations and the excretion of 24 h microalbuminuria(UAE) were examined,and the expressions of TNF-α,TLR4 and NF-κB-p65 in the renal cortex and glomerular microstructure changes were observed by light microscope after the experiment end. The results indicated that group DM had pathological changes of glomerular capillary loop increased and dilation of renal tubule and its epithelial cell epithelial and degeneration. In addition,cell aggregation increased and infiltration of inflammatory cells,while the above glomerular injury improved significantly in groups of DE,DS and DES with exercise and spirulina supplement through light microscope. Compared with group DM,the concentrations of blood glucose and 24h UAE in the intervention groups decreased significantly(P<0.05 or P<0.01),and that improved significantly after two interventions than the single intervention. Compared with group DM,the expressions of NF-κB-p65 and TNF-α,TLR4 significantly decreased in group DE of the renal cortex(P<0.05 or P<0.01),and that improved significantly after two interventions than the single interventions. Therefore,exercise and/or spirulina supplement can improve blood glucose and renal function,and decrease renal injure for type 2 diabetes. Combination of swimming and spirulina supplement showed stronger effects on protein expression of TLR4/NF-κB-p65 signal which had anti-inflammatory effect than either sole swimming or sole spirulina supplement.

Key words: type 2 diabetes, exercise training, renal injury, TLR4/NF-&kappa, B-p65, spirulina