Abstract: Zanthoxylum nitidum is a woody liana plant with high value of medicine,but its resources is shortage.To recover the resources of Z.nitidum, germchits should be obtained by tissue culture.The results showed that the optimal medium for callus induction from explant of sterile stem segment was MS+4.0 mg/L KT+2.0 mg/L NAA with induction rate of 74.84%.The optimal medium for bud differentiation was MS+6.0 mg/L KT+0.2 mg/L IBA.The bud differentiation rate was 36.46% after two times of differentiation culture,and 3.30 buds were regenerated from a piece of callus.The quantity and quality of regenerated bud could be improved and increased by proliferation culture.The optimal medium for bud proliferation was MS+4.0 mg/L KT+0.8 mg/L NAA,the rate of bud proliferation reached 5.00 after 8 weeks culture.The optimal medium for root differentiation was 1/2MS+0.2 mg/L KT+1.0 mg/L IBA with highest differentiation rate of 66.75%.A tissue culture system with high proliferation rate was established by successfully differentiating buds and roots from calli of Z.nitidum.

Key words: Zanthoxylum nitidum, tissue culture, explant, callus, differentiation