Abstract: Fifty-four SD rats were randomly divided into 3 groups:control (CK),general tobacco (GT) and new-type tobacco (NT).The COPD model was duplicated in cigarette smoke-exposed rats in order to reveal the biological effect of tobacco introduced Perilla frutescents (L.) Britt genes.ELISA analysis showed cigarette smoke can induce inflammatory response of COPD rats after 56 d exposure.Compared to GT group,the serum levels of CRP,IL-8 and TNF-α in NT group significantly decreased (P<0.05).The expression of NF-κB p65 in liver and spleen tissues was detected by immunohistochemical method.Compared to CK group,there was a significant increase in their levels in COPD rats.With the extension of exposure time,there were obvious differences between NT and GT group (P<0.05).The results indicated that the new-type tobacco can suppress the systemic inflammatory of smoking-related COPD and intervene in the overactivation of NF-κB in extrapulmonary tissues,resulting in delaying the progression of the disease.

Key words: chronic obstructive pulmonary disease, Perilla frutescents (L.) Britt, cigarette smoke, systemic inflammation