Abstract: In this study,ultrasonic-assisted stepwise biocatalysisextraction was used to extract dioscin from Trigonell afoenum-graeeum. The extraction conditions, including enzyme dosage,temperature,pH and time,were optimized usingthe extraction yield of dioscin as evaluation indexby using single factor experiment and response surface methodology.Results showed that the extraction yield of dioscinunder the optimal condition was 2.29%,36.3% higher than ultrasonic.It wasan effective,simple method to separate dioscin from T.afoenum-graeeum by ultrasonic-assisted stepwise biocatalysis extraction.

Key words: Trigonell afoenum-graeeum, dioscin, stepwise biocatalysis, ultrasonic extraction, response surface methodology