Abstract: This experiment was done by using HPLC combined with CAD.Hypersil GOLD C₁₈ was used as the chromatographic column.The mixture solution of Nonafluoropentanoic acid (0.05% in water) and methanol with a proportion of 97∶3 was used as the mobile phase for the isocratic elution.Determination of GABA content in compound aminobutyric acid and vitamin E capsule was done by comparison of the described curve and equation of the standard GABA compound.The Linear equation in this experiment was described as y=-0.00004x²+0.0602x+1.6924,r=0.9997,with the linear range of 52~415 μg*mL^-1.The mean recovery of samples was 101.6% and RSD% was 1.9% (n=9).This method is simple and convenient,which could give a rapid determination of GABA content in aminobutyric acid vitamin E capsule.

Key words: aminobutyric acid vitamin E capsule, GABA, CAD, HPLC