Abstract: The purpose of the present work was to clarify the chemical constituents of the leaves of Chinese chive (Allium Tuberosum) which except for the volatile oil.The leaves were extracted with 95% ethanol,and the compounds were isolated and purified by macroporous resin,gel column chromatography and preparative high performance liquid chromatography.The structure of the compounds was determined by nuclear magnetic resonance.A total of 12 compounds were isolated from the chive leaves,and the structures were identified as:adenine（1）,p-methylphenol（2）,tryptophan（3）,lignan-8-O-4′-glucoside（4）,3-［(2S，3R)-2,3-dihydro-2-(4-hydroxy-3- methoxyphenyl)-3-(hydroxymethyl)-7-methoxy-5-benzofuranyl］propyl-β-D-Glucopyranoside（5）,kaempferol-3-O-sophoroside-7-O-β-D-glucopyranoside（6）,3-［2-［4-(β-D-glucopyranosyloxy)-3-methoxyphenyl］- 2,3-dihydro-3-(hydroxymethyl)-7-methoxy-5-benzofuranyl］propyl-(2-trans)-β-D-Glucopyranoside（7）,kaempferol（8）,7-［(4-O-β-D--Glucopyranosyl-β-D-glucopyranosyl)oxy］-3,5-dihydroxy-2-(4-hydroxyphenyl)-4H-1-benzopyran-4-one（9）,adenosine（10）,quercetin（11） and ferulic acid（12）.Compounds 2~9,11~12 were isolated form Allium Tuberosum for the first itme The content of flavonoids is more than other compounds.The results provide a theoretical basis for the further determination of nutrient components in chive,and which provide a reference for the study of chemical composition of Allium Tuberosum.

Key words: leaves of Chinese chive, structural identification, preparative high performance liquid chromatography(RP-PHPLC), flavonoids