Abstract: A simple and rapid detection method was established by liquid chromatography-tandem mass spectrometry to study the residues of 10 androgen and assimilation hormones in mutton.Acetonitrile was used as the extractant,extracted by QuEChERS technique,purified by purification powder,and determined by liquid chromatography-tandem mass spectrometry.Optimized by chromatographic conditions,the best chromatographic conditions were:gradient elution with acetonitrile-0.1% formic acid as mobile phase,flow rate of 0.25 mL·min^-1,column temperature of 30 ℃,injection volume of 5 μL,10 species The androgen and anabolic hormone peaks are sharp and sensitive.The detection limit of this method is 1 ng·kg^-1,the limit of quantification is 100~300 ng·kg^-1,R² is greater than 0.999,and the linear relationship is good.The average recovery is 51.36%~91.34%,and the RSD is below 3%.This method was used to measure the mutton of Lanzhou Slaughterhouse.The 10 kinds of androgen and assimilation hormones were not detected,indicating that the breeding process is strict,the method is simple,rapid,sensitive,and can quantitatively and qualitatively analyze various hormones.

Key words: liquid chromatography-tandem mass spectrometry, mutton, androgen, anabolic hormone