Abstract: In this study,an immortalized mouse myeloid cell line was prepared.The Tet-On inducible retrovirus vector was used to induce the expression of MLL-AF9 fusion gene in hematopoietic progenitor cells/stem cells of adult bone marrow by adding Doxycycline to obtain a myeloid cell line.This study found that immortalized mouse myeloid cell line preparation methods,through the conditional expression of MLL-AF9 obtained immortalized myeloid cell lines compared with the primary myeloid cells in adult mouse bone marrow,have a similar surface The marker,ie,CD11b⁺,but immortalized myeloid cell line is easily amplified,obtaining a large number of uniform cell populations.

Key words: Myeloid cell line, virus, Preparation, Doxycycline