NATURAL PRODUCT RESEARCH AND DEVELOPMENT ›› 2018, Vol. 30 ›› Issue (增刊2): 64-74.

• Article • Previous Articles     Next Articles

Simultaneous Determination of 7 Effective Components Content in Herbal Artemisia annua by UPLC-MS

DING Gang1,2,3,SONG Xu-hong1,2,3,CUI Guang-lin1,2,3,TAN Jun1,2,3,LI Long-yun1,2,3*   

  1. 1Chongqing Academy of Chinese Materia Medica; 2Chongqing Engineering Research Center for Fine Variety Breeding Techniques of Chinese Maeria Medica; 3Chongqing Sub-center of National Resource,Center for Chinese Materia Medica,China Academy of Chinese Medical Scicne,Chonqing 400065,China
  • Online:2019-01-18 Published:2019-01-18

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Abstract: Establishing a UPLC-MS method for rapid determination of artemisinin,artemisinic acid,artemisinin,dihydroartemisinin,kaempferol,luteolin and quercetin in Artemisia annua L.Samples extracted by ultrasound.Chemical composition was detected bymass spectrometer (AB SCIEX TRIPLE QUAD 5500),andquantifiedby peak area external standard method.The calibration curves of artemisinin,artemisinicacid,annuaHB,dihydroartemisinin,kaempferol,luteolinand quercetin were in good lineafity respectively,and the average recoveries were 99.14%(RSD=1.84%),97.22%(RSD=2.67%),98.22% (RSD=2.04%),101.47%(RSD=2.53%),97.34%(RSD=3.02%),101.51%(RSD=256%),100.51%(RSD=3.03%),respectively.The measurement from 50 locations in Chongqing showed that the method is simple and accurate.It can be used for quality evaluation of Artemisia annua.

Key words: Artemisia annua, artemisinin, artemisinicacid, annuaHB, dihydroartemisinin, UPLC-MS

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