NATURAL PRODUCT RESEARCH AND DEVELOPMENT ›› 2018, Vol. 30 ›› Issue (11): 1963-1970. doi: 10.16333/j.1001-6880.2018.11.019

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Analyses and Genetic Variation of rDNA ITS in Ophiocordyceps sinensis from Different Geographical Regions

WANG Jia-chun1, XU Jun2, LI Zhao-lan3*, WANG Yue4, CHEN Ya-lin1   

  1. 1PLA Navy Medical research institute,Shanghai 200433,China; 2Shanghai Health Medical College,Shanghai 200093,China; 3College of Life Science,Nanjing University,Nanjing 210093,China; 4College of Life Science and Technology,Xinjiang University,Wulumuqi,830046,China
  • Online:2018-12-03 Published:2018-12-03

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Abstract: To investigate the genetic variation among Ophiocordyceps sinensis from different geographical regions,the sequence of 18S rDNA ITS was analysed in Ophiocordyceps sinensis,in order to establish the molecular genetic marker for O.sinensis from different geographical regions.Total DNA of dry specimens was extracted from stroma or sclerotium powder of O.sinensis using a modification of the DNA isolations protocol of Lee＆Taylor (1990).The crude extracts were examined by 1% agarose gel electrophoresis in 1×TAE buffer.The extracts were used as templates and were amplified with PCR technology,PCR amplification products were purified,sequenced and compared.The present study showed that sequences of 18S rDNA ITS there were obvious genetic variations and dissimilarity among in O.sinensis from different geographical regions,such as,sequences composition,GC content (%) etc.The results showed that genetic differentiation of O.sinensis has close relation to latitudes and elevation highness.These differences can be used to differentiate locality identificiation of O.sinensis.

Key words: Ophiocordyceps sinensis, rDNA ITS, PCR amplification, genetic differentiation, elevation highness and latitudes

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