Isolation and Identification of Huperzine A-producing Endophytic Fungus SNZ-12 from Huperzia serrata

doi:10.16333/j.1001-6880.2018.12.015

Abstract

Abstract: To obtain Huperzine A-producing endophytes,sixty endophytic fungi were isolated from Huperzia serrata in this study,and the extract of each of the fungi was detected by HPLC technology and MS technology.The extract of one endophytic fungus numbered as SNZ-12 had the same chromatographic peak and retention time as that of authentic Huperzine A (8.994 min),as well as contained the same mass peak with authentic Huperzine A((M+H)⁺=243.1),indicating that the endophytic fungus SNZ-12 could produce Huperzine A.The Huperzine A yield of the endophytic fungus SNZ-12 was about 1.01 mg/L.Based on the morphological characteristics and 18S rDNA sequence conservation of the endophytic fungus SNZ-12,it was identified as Fusarium oxysporum.Our results provided a potential strain for the production of Huperzine A through microbial fermentation.

Key words: Huperzia serrata, endophytes, Huperzine A, Fusarium oxysporum

CLC Number:

R284.1
Q93

ZHANG Hong-qing, ZHANG Tian, ZHANG Xue-zhi, XU Wen-jing, LI Hao-dong, LIU Pan, ZHANG Peng*, SONG Fa-jun. Isolation and Identification of Huperzine A-producing Endophytic Fungus SNZ-12 from Huperzia serrata[J]. NATURAL PRODUCT RESEARCH AND DEVELOPMENT, 2018, 30(12): 2133-2137.

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Isolation and Identification of Huperzine A-producing Endophytic Fungus SNZ-12 from Huperzia serrata

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