Abstract: Using the training model of mice running and the methods of immunoprecipitation and immunoblotting,this study aimed to investigate the protective effects of verbascoside on skeletal muscle damage by incremental load exercise and glutathione of mice.Ninety mice were divided into six groups including:the normal group (A),the normal + verbascoside treated group (B),the exercise group(C),the exercise + verbascoside treated by the low dosage (D),the exercise + verbascoside treated by the moderate dosage (E),the exercise + verbascoside treated by the high dosage (F).The results showed that the degree of skeletal muscle damage decreased in turn in the three groups of exercise+verbascoside (D、E、F) compared with group C.The skeletal muscle morphology of the mice in group F was almost normal.The plasma CK levels and skeletal muscle tissue GSSG content in group F were lower than those of groups of C、D、E (P<0.01).The plasma CK levels in group F were higher than those of groups of A and B (P<0.01),but there is no significant difference in the skeletal muscle tissue GSSG content among group F、A and B (P>0.05).The GSH contents,GSH/GSSG ratios,the enzyme activities of GCL and GR in skeletal muscle tissue,the levels of protein expression of GSH in RyR1 complexes of mice in group F were higher than those of groups C、D、E,but were lower than those of group A and B (P<0.01).The results suggested that verbascoside can reduce the levels of plasma CK,protect the morphology of the skeletal muscle of mice exercised by incremental load.Its mechanism was related to verbascoside increasing of GSH contents and the enzyme activities of GCL and GR in skeletal muscle,improving the levels of protein expression of GSH in RyR1 complexes and decreasing the ratios of GSSG/GSH in skeletal muscle of mice exercised by incremental load.

Key words: verbascoside, exercise, skeletal muscle, glutathione, RyR1