Abstract: To establish a UPLC method for the simultaneous determination of tectoridin,iristectorin A,iristectorin B,iridin,tectorigenin,iristectorigenin A,iristectorigenin B in Iris tectorum Maxim..Waters BEH C₁₈(2.1 mm×50 mm,1.7 μm) column was used,with acetonitrile-0.5% glacial acetic acid as the mobile phases in gradient elution mode.The column temperature was 35 ℃,with flow rate 0.5 mL/min.The UV detection wavelength was set at the maximum absorption wavelength,266 nm for all components.The seven kinds of isoflavone components in I.tectorum were separated well,and the linear relationship was obtained (r≥0.9990).The average recoveries were 99.68%,100.57%,98.43%,98.03%,99.14%,97.53% and 101.86%,with RSDs were 0.84%,1.82%,1.67%,1.54%,0.89%,1.95% and 1.77%,respectively.The established method was rapid,simple and accurate,it can provide the basis for the quality control of I.tectorum.

Key words: isoflavone, Iris tectorum Maxim., quality control, UPLC