Abstract: In this study,the anti-tumor mechanisms of Radix Puerariae were illustrated by network pharmacology method.First,the active compounds were screened out from Radix Puerariae using drug-likeness (DL) evaluation approach.Second,the anti-tumor related protein targets were predicted by a reverse docking tool,and hand-searched in Pubmed database.Then,potential targets were submitted to MAS3.0 database and KEGG database to predict related pathway.Finally Cytoscape 3.3 software was used to construct "compounds-targets","targets-pathways" and "compounds-targets-pathways" networks.For Radix Pueraria,results showed that 16 active compounds,34 potential targets and 44 signaling pathways may participate the anti-tumor activities.Network analysis showed active compounds could affect directly on many type of cancers,or indirectly with tumor-related signaling pathway.In summary,through this study,anti-tumor mechanisms of Radix Puerariae demonstrate the features of traditional Chinese medicine (TCM) in a "multi-compounds,multi-targets and multi-pathways" mode.These provide the possible direction for the following pharmacological experiments,and gives lessons for other TCM researches.

Key words: Radix Puerariae, network pharmacology, cancer, protein target, action mechanism