NATURAL PRODUCT RESEARCH AND DEVELOPMENT ›› 2018, Vol. 30 ›› Issue (9): 1509-1514. doi: 10.16333/j.1001-6880.2018.9.006

• Article • Previous Articles     Next Articles

Isolation and Identification of a Salt-tolerant Yeast in Pixiandouban and Its Fermentation Characteristics

ZHANG Qian1,2#, YANG Tao1#, YAO Chan1,2, LI Guo-you1, WU Lin-wei1, FANG Dong-mei1, HUANG Tian-fang1*, CHEN Xiao-zhen1*   

  1. 1Chengdu Institute of Biology,Chinese Academy of Science,Chengdu 610041,China; 2University of Chinese Academy of Sciences,Beijing 100049,China
  • Online:2018-10-08 Published:2018-10-10

Abstract: A salt-tolerant yeast strain HJ-7Y.1 was isolated from Pixiandouban which was identified as Zygosaccharomyces rouxii by morphological,physiological,biochemical and ITS rDNA analysis.The salt tolerance test was found that HJ-7Y.1 grew well in medium containing 260 g/L NaCl,which showed HJ-7Y.1 had high salt tolerance.The gas chromatography(GC)and headspace solid-phase microextraction/gas chromatography-mass spectrometry(HS-SPME/GC-MS)techniques were used to determine the content of ethanol and the volatile aroma compounds in the fermentation broth of the strain.The results showed that HJ-7Y.1 had good ethanol production ability under high salt concentration,and could produce various aromatic components,such as phenyl ethanol,ethyl acetate,isoamyl alcohol and phenylethyl acetate,which could bring positive effects to the characteristic flavor of Pixiandouban.Therefore,this strain of Zygosaccharomyces rouxii has potential further application in Pixiandouban production.

Key words: Pixiandouban, Zygosaccharomyces rouxii, salt tolerance, ethanol, aroma components

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