Abstract: To evaluate the quality of Panax notoginseng Fruit stems in different areas,the HPLC method was established to determine the contents of six components inPanax notoginseng Fruit stems such as ginsenosides Rb₁,Rc,Rb₂,Rb₃ and notoginsenosides Fa,Fc.The conditions were as follows,Thermo Scientific Hypersil GOLD C₁₈ column (250 mm×4.6 mm,5 μm),flow rate:1.0 mL/min,detection wavelength:203 nm,injection volume:10 μL.This method showed a good linearity with the range of 0.7813-3.0000×102 μg/mL.The average recovery of Rb₁,Rc,Rb₂,Rb₃,Fa and Fc was respectively 102.33% (RSD =2.82%),97.29% (RSD =4.15%),97.41% (RSD =2.37%),98.65%(RSD=1.96%),96.13%(RSD=2.35%)and 97.83% (RSD =4.87%).The contents of six components in Panax notoginseng Fruit stems areRb₃>Fc>Rb₁>Fa>Rc>Rb₂,and it showed some differences by area and year,ranging from 2.11% to 3.84%.The method was simple and reliable,and could be used in the quantitative determination of saponins and development in Panax notoginseng fruit stems.

Key words: Panax notoginseng fruit stems, different areas, saponins, HPLC, content determination