Abstract: A sensitive high performance liquid chromatography-tandem mass spectrometry (HPLC-MS/MS) method was developed for the determination of frangufoline (sanjoinine A) from Semen Zizyphi Spinosae.The reflux extraction conditions (extraction solvent,the acid in extraction solvent,extraction temperature,acid concentration,liquid-solid ratio,extraction time) of frangufoline from Semen Zizyphi Spinosae were investigated by single factor tests and orthogonal experiment with the extraction rate of frangufoline as the index.The results showed that the optimal extraction solvent was ethanol and the optimal acid was acetic acid.The optimal extraction conditions were as follows:extraction temperature 60 ℃,liquid-solid ratio 20∶1 (V∶g),acetic acid concentration 8%,extraction time 4 h.Under the optimal extraction conditions,the extraction rate of frangufoline was 32.286 μg/g.

Key words: Semen Zizyphi Spinosae, frangufoline, HPLC-MS/MS, orthogonal experiment