Abstract: In order to prepare drug loading transfersomes (Sophora flavescens Ait.alkaloids nano-liposomes) with Sophora flavescens Ait.alkaloids,as model,and to optimize their preparation technology.The optimal technology conditions of the transfersomes are as follows:phospholipid concentration is 2.75%,the ratio of PC/CH is 3∶1,1,2-Propanediol concentration is 0.5%,and aquation time is 34.4min.The results show that the encapsulation efficiency of the transfersomes are (81.15±1.63)%（n=3）under the verification test.And there are small deviations between the measured values of index and model predicted values.It shows that the response surface analytical method is reliable and can be used in the process of prescription optimization,and the technique of preparing the Sophora flavescens Ait. alkaloids transfersomes is feasible,convenient and reliable in quality.

Key words: Sophora flavescens Ait. alkaloids, reverse evaporation method, transfersomes, embedding ratio, response surface methodology