Abstract: Casein hydrolysate (CH),LH35 whey protein hydrolysate (LH35) and LH80 whey protein hydrolysate (LH80) were prepared by trypsin hydrolysis of casein,LH35 whey protein and LH80 whey protein,respectively.Hyclone hydrolyzed milk protein (HLH) was used as a control to study the effects of different hydrolyzed milk proteins on the growth and metabolism of BHK-21 suspension cells.The results showed that when 2g/L hydrolyzed milk protein was added to the medium,CH,LH35,LH80 and HLH had a growth-promoting effect on BHK-21 suspension cells,among which LH35 and LH80 had better growth promoting effects than HLH,and CH and HLH had similar growth promoting effects.Hydrolyzed milk proteins have different effects on the biochemical,enzymatic and amino acid metabolism of BHK-21 suspension cells.This lays the foundation for the application of protein hydrolysates in cell culture.  

Key words: hydrolyzed milk protein, BHK-21 suspension cells, biochemical metabolism, amino acid metabolism