Abstract: In order to investigate the effect of n-butyl alcohol extract of Toona sinensis (NBAE) on the inflammation of glomerular endothelial cells in diabetic nephropathy (DN) and to explore the possible mechanisms,Wistar male rats were injected with STZ.After successful modeling,STZ rats were divided into DN group and DN+NBAE intervention group.After 8 weeks,the rats were sacrificed which blood sample were tested for BUN and Scr and the kidneys were collected for HE staining and PAS staining.MCP-1,ICAM-1 and phosphorylation of p65 were detected by immunohistochemistry staining.After stimulating glomerular endothelial cells in vitro with 25 mmol/L high glucose (HG),HG+NBAE,and HG+NF-kB p65 blocker PDTC,MCP-1,ICAM-1 and phosphorylated p65 were detected by Western blot.The results showed that compared with the DN group,the blood glucose was significantly reduced and the injury was alleviated in DN+NBAE group.Otherwise,MCP-1,ICAM-1 and phosphorylated p65 were all reduced.In vitro,NBAE significantly decreased the expression of MCP-1 and ICAM-1 (P<0.01),which were similar with the treatment of NF-kBp65 blocker PDTC.In conclusion,it can be proved that endothelial inflammation of DN can be alleviated by NBAE,which is presumably related to inhibition of NF-kB signaling pathway.

Key words: diabetic nephropathy, inflammation, Toona sinensis extract, NF-kB signaling pathway, glomerular endothelial cells