Abstract: Four sesquiterpenoids,named phomophyllin O (1),phomophyllin P (2),7-hydroxy-10-methoxydehydrodihydrobotrydial (3),and plorantinone D (4),and eight steroids,named fortisterol (5),dankasterone B (6),(14α,22E)-14-hydroxy-ergosta-7,22-diene-3,6-dione (7),(14α,22E)-14-hydroxyergosta-4,7,22-triene-3,6-dione (8),calvasterol B (9),isocyathisterol (10),ergosta-4,6,8(14),22-tetraen-3-one (11),and ganodermaside D (12),were isolated from the ethyl acetate extract of the solid-state fermentation of the endophytic fungus Phomopsis sp.TJ507A,a strain isolated from the medicinal plant Phyllanthus glaucus.These compounds were purified by comprehensive chromatography on silica gel column,Sephadex LH-20,octadecyl silane (ODS),and semi-preparative HPLC.Two new sesquiterpenoids 1 and 2 were identified and their structures were determined by detailed analyses of the NMR spectra,［Rh₂(OCOCF₃)₄］-induced ECD spectra,and comparison with literatures.Compounds 1-5,7,8 and 10 were isolated from the genus Phomopsis for the first time.Evaluation of in vitro anti-inflammatory activity of the isolates showed that compound 9 exhibited significant NO inhibitory activity with the IC₅₀ value at 8.7 μM.

Key words: endophytic fungus, Phomopsis sp., sesquiterpenoids, steroids, biological activity