Abstract: In order to study the tissues distribution of the seven components from Inula cappa extract in rat after the administered intragastrically,UPLC-MS /MS for the simultaneous determination of neochlorogenic acid,chlorogenic acid,cryptochlorogenic acid,1,3- two caffeoyl quinic acid,3,4- two caffeoyl quinic acid,4,5- two caffeoyl quinic acid,and galuteolin in rat tissues were established.Rats were administered intragastrically Inula cappa extract,and main tissues and organs were collected from rats at 0.5,1.5,and 5 h after administration.After pretreatment,the concentrations of neochlorogenic acid,chlorogenic acid,cryptochlorogenic acid,1,3- two caffeoyl quinic acid,3,4- two caffeoyl quinic acid,4,5- two caffeoyl quinic acid,and galuteolin in each organ of each time point were determined.After being administered Inula cappa,for neochlorogenic acid,its concentration peaked in the small intestine,kidney,lung and liver at 0.5 h;peaked in the stomach,muscle and spleen at 1.5 h;peaked in the heart at 5 h.For chlorogenic acid,its concentration peaked in the small intestine,kidney,lung and heart at 0.5 h;peaked in the stomach,muscle,spleen and liver at 1 h.For cryptochlorogenic acid,its concentration peaked in the small intestine,kidney,lung at 0.5 h;peaked in the stomach,muscle,spleen,heart and liver at 1 h.For 1,3- two caffeoyl quinic acid,its concentration peaked in the small intestine,kidney,lung and heart at 0.5 h;peaked in the stomach,muscle,liver and spleen at 1.5 h.For 3,4- two caffeoyl quinic acid,its concentration peaked in the small intestine and kidney at 0.5 h;peaked in the stomach,muscle,liver and spleen at 1.5 h;peaked in the heart and lung at 5 h.For 4,5- two caffeoyl quinic acid,its concentration peaked in the small intestine heart and kidney at 0.5 h;peaked in the stomach,muscle,liver and spleen at 1.5 h;peaked in the lung at 5 h.For galuteolin,its concentration peaked in the small intestine and heart at 0.5 h;peaked in the stomach,liver and spleen at 1.5 h;peaked in the lung and kidney at 5 h.After the rats administered instragastrically Inula cappa,its seven components could be widely and quickly distributed in various tissues and organs;however,there are differences in the peak time and distribution of these seven components,these seven components were not detected in brain tissue.Seven components are mainly distributed in the stomach,small intestine and kidney tissue,showing a strong affinity for the kidney,it is speculated that the kidney may be one of the main excretory organs of feverfew.

Key words: Inula cappa extract, tissue distribution, 4,5-two caffeoyl quinic acid, neochlorogenic acid, chlorogenic acid