Abstract: To investigate the osteogenesis ability of NPP/C-HA composite scaffold by in vivo experiments.The distal bone defect model (diameter 7mm,depth 10mm) of bilateral rabbit femur was used to observe the repair effect of bone defect by gross specimen,imaging examination,molecular biology examination and histological examination.No obvious adverse tissue reaction was observed in each group.With the increase of observation time,As the observation time increases,the bone defect area of the experimental group gradually becomes smaller,The BALP content of the experimental group at 4 weeks,6 weeks,and 8 weeks exist statistically significant compared with other groups;in the experimental group,more new bone appeared at the edge of the defect area,but there was no significant difference in bone maturity.The results showed that NPP/C-HA scaffold has good biocompatibility and positive osteogenic effect,which provides an experimental and theoretical basis for further study of the application of NPP/C-HA in bone tissue engineering.

Key words: nanometer pearl powder, chitosan, hyaluronic acid, scaffold, bone defect