Abstract: This research aims to investigate the active constituents of Morinda officinalis How and its anti-fatigue effect on mice.The active constituents was extracted by ethanol reflux,separated and purified by macroporous resin,MCI,and ODS column chromatography,respectively.The structure of the obtained product was identified by NMR.Results showed that 10% ethanol eluate from the macroporous resin extracted from the Morinda officinalis How had the most obvious anti-fatigue effect on mice.After separation by MCI and ODS column,four compound monomers were further separated： nystose(1),4-carboxyl-7-hydroxyl-8-hydroxymethyl-3,6-iridoid glycosides(2),4-acetyl-8-hydroxymethyl-3,7-iridoidglycosides(3),4-carboxyl-7-hydroxyl-8-hydroxymethyl-10-acetyl-3,6-iridoid glycosides(4).Compounds 2,3 and 4 are firstly isolated and identified from Morinda officinalis How,which will provide a theoretical basis for clarifying the exploitation value of the resource to be developed into healthcare products.

Key words: Morinda officinalis How, anti-fatigue, isolation, structural identification