Abstract: The aim of this study was to investigate the mechanism of BPIS (bound phenol of inner shell) against breast cancer cell. Firstly, the effect of BPIS on the activity of breast cancer cells and normal breast cells was detected by cell counting. Secondly, the SEA, SIB and GeneCards databases were used to predict and screen the targets of BPIS on breast cancer. Then, the String database, Cytoscape software and DAVID database were used to construct the active components-targets network and analyse pathway. After these studies, 39 targets were screened, which involved in biological processes such as Glycolipid metabolism, autophagy and so on, and signaling pathways such as MAPK, PI3K/AKT, FoxO and so on. These results indicated that BPIS anti-breast cancer is a multi-component, multi-target, multi-signal synergistic process, and autophagy associated with cell death is likely to play a major role in BPIS inhibition of breast cancer.

Key words: BPIS, breast cancer, network pharmacology, pharmacological mechanism