Abstract: To explore the protective effects of esculin on vascular endothelial cell line,the effect of esculin on the proliferation of human umbilical vein endothelial cells cultured in vitro by AGEs was evaluated by CCK-8 assay.The effects of different concentrations of AGEs and esculinon on the levels of nitric oxide (NO) and asymmetric dimethylarginine (ADMA) in endothelial cells were examined.Endothelial cells oxidative stress related indicators were detected,including superoxide dismutase detection (SOD),reactive oxygen species (ROS),and malondialdehyde (MDA).Lactic dehydrogenase (LDH),total cholesterol (CHO),triglyceride (TG) and low density lipoprotein (LDL) were also detected.The levels of adhesion-related factors:vascular cell adhesion molecule-1 (VCAM-1) and intercellular adhesion molecule-1 (ICAM-1) were also evaluated.The result indicated that 200 mg/L AGEs significantly inhibited the proliferation of human endothelial cell ECV304.The esculinon could inhibit the proliferation of endothelial cells induced by AGEs,and it was concentration-dependent.At 25 mg/L,the protective effect was the highest.The oxidative stress index ROS results suggested that esculinon could resist AGEs-induced ROS production and improve liquid metabolism:cholesterol,LDL and TG levels were significantly improved after the treatment with esculinon.The esculinon could significantly inhibit the expression of the endothelial adhesion factor VCAM-1.The expression of NO was increased and the level of ADMA was deceased.Esculinon could effectively promote the proliferation of human vascular endothelial cell and had potential effects on endothelial cells in improving ROS,lipid metabolism and adhesion factors and NO releasing.

Key words: esculin, human umbilical vein endothelial cells, glycation end products, proliferation, oxidative stress, adhesion factor