The constituents of ethanol extract of M. crystallinum were isolated and purified with varied chromatographic techniques.All isolated compounds were identified on the basis of NMR data and physicochemical properties.Nine compounds were obtained and identified as dibutyl phthalate (1),ixerol B (2),cyclo-(Phe-Val) (3),(1S,3S)-1-methyl-1,2,3,4-tetrahydro-β-carboline-3-carboxylic acid (4),(1R,3S)-1-methyl-1,2,3,4-tetrahydro-β-carboline-3-carboxylic acid (5),jasmioside E (6),uridine (7),vitamin B4 (8) and syringin (9).All aforementioned compounds were isolated from M. crystallinum for the first time.This study enriched the chemical constituents of this plant.All above compounds showed no inhibitory activities towards human cervical carcinoma (Hela),human gastric adenocarcinoma (SGC-7901),human alveolar epithelium (A549),human hepatoma (BEL-7402) and chronic myelogenous leukemia (K562) cell lines.