To investigate the chemical components of Trevesia palmate and evaluate their cytotoxicity,the chemical constituents of the methanol extract of T. palmate were separated and purified by chromatographic techniques,including column chromatography of silica gel,Sephadex LH-20,Rp-C₁₈ and HPLC. Their structures were mainly elucidated by NMR and MS spectroscopic techniques. Fourteen compounds were isolated and purified.They were identified as hederagenin 3-O-α-L-rhamnopyranosyl-(1→2)-α-L-arabinopyranoside (1),oleanic acid (2),ursolic acid (3),dipsacoside B (4),macranthoidin B (5),hederacoside C (6),momordin Ic (7),ferulic acid (8),salicin (9),chlorogenic acid (10),rutin (11),β-adenosine (12),β-sitosterol (13) and palmitic acid (14).Compounds 2-14 were isolated from T. palmate for the first time. Some compounds were tested for their cytotoxicities against human tumor cell lines SMMC-7721,Hela and MCF-7,and compounds 1 and 3 exhibited inhibitory effects on all three cellsThe cytotoxicity of compound 1 on Hela was reported for the first time.