The antimicrobial effect of iturin A on Fusarium fujikuroi and Botrytis cinerea was studied.The sodium dehydroacetate and natamycin were used as positive controls.The results showed that the minimum inhibitory concentration (MIC) of sodium dehydroacetate,natamycin,and iturin A for F. fujikuroi, were 3,7.5 and 0.051 8 mg/mL,respectively.For B. cinerea,the MIC of sodium dehydroacetate,natamycin,and iturin A were 20,20 and 0.037 0 mg/mL,respectively.These results indicated that both F. fujikuroi and B. cinerea were more sensitive to iturin A than sodium dehydroacetate and natamycin.The nucleic acid and protein leakage were measured for the spoilage mold treated after the three bacteriostatic agents.The results showed that all the three bacteriostatic agents could destroy the cell membrane of the spoilage mold,which led to the leakage of nucleic acid and protein,thereby achieving the antibacterial effect.In this paper,possible data on antibacterial effect of iturin A on the spoilage mold of postharvest strawberries in this study might provide a theoretical basis for the use of iturin A on postharvest preservation of strawberries.