Abstract: This study was designed to elucidate the effect of the tea seed saponin on intestinal microflora of hyperlipidemic rats.After a week adaptive feeding,60 male Wistar rats were randomly divided into normal group (10 rats,group C) and model group (50 rats),which were fed with basic diet and high fat diet.After successful modeling,the model rats were randomly divided into group H,group P,group TSL,group TSM,and group TSH according to their body weight,and were intragastrically administered.Rat intestinal microflora was detected using 16S rRNA sequencing technology after 4 weeks of intervention.The results showed that the richness and diversity of intestinal microflora in group H were significantly decreased,and the community structure changed significantly from gate to genus compared with group C.After the application of tea seed saponin,the abundance of intestinal microflora and the community structure from the door to the genus were also effectively regulated,the relative abundance of beneficial bacteria increased,and the pathogenic bacteria were inhibited.The results indicated that tea seed saponin plays a positive role in regulating the intestinal microflora of hyperlipidemic rats induced by high fat diet.

Key words: tea plant, teaseed saponin, hyperlipidemia, intestinal microflora