Abstract: To investigate the effects of polysaccharide components FOPS-a and FOPS-b from Fomes officinals Ames on the expression of AKT/GSK3β/Tau/P-tau in hippocampus of Alzheimers disease model mice.The sixty-four male APP/PS1 double transgenic AD model mice were randomly divided into 8 groups as follows:model group,donepezil group (0.65 mg/kg),the high,medium and low doses of FOPS-a and FOPS-b groups(60,30,15 mg/kg) with 8 mice in each group.The same age of wild type C57BL/6J mice were included as normal group.Mice were treated with drugs and saline administered by intragastric administration for 90 days.At 6 months of age,the behavioral changes of mice were detected by jumping platform and open field test.The morphological changes of neurons in hippocampus of each group were observed by Nissl staining.RT-PCR was used to detect the transcription levels of AKT,GSK3β  and Tau mRNA in hippocampus of mice.Western-blot method was used to detect AKT,GSK3β,Tau,and P-tau protein expression.The study was found that the FOPS-a and FOPS-b can significantly improve the learning and memory function of mice and protect neurons from damage in the hippocampus,up-regulates AKT mRNA transcription levels and protein expression,and down-regulates GSK3β,Tau mRNA transcription levels and GSK3β,Tau,P-tau protein expression.Studies have shown that the FOPS-a and FOPS-b can protect neurons from damage by reducing the tangles of neuron fibers in the hippocampus and play an antagonistic role in AD.

Key words: polysaccharide components from Fomes officinals Ames, Alzheimer's disease, AKT, GSK3&beta, , Tau