Abstract: To establish a rapid method for the determination of deltonin,zingiberensis newsaponin and dioscin in Dioscorea zingiberensis C.H.Wright.The research objects were the medicinal materials of D. zingiberensis from 8 producing areas in China.Firstly,using HPLC-ELSD established the method of simultaneously determining the contents of deltonin ,zingiberensis newsaponin and dioscin,which could be used to determine the contents of three saponins in D. zingiberensis from different habitats .Secondly,the samples were scanned for obtainning their near infrared spectrums,the contents of three saponins in the calibration set samples of D. zingiberensis were taken as reference values respectively,combined with their near-infrared spectrums,the internal cross-validation determination coefficient (R²),corrected root mean square deviation (RMSEC),predicted root mean square deviation (RMSEP) and predicted performance index (PI) were used as indicators to evaluate the performance of the quantitative test model.TQ 8.0 analysis software were combined with partial least squares (PLS) to establish a rapid detection model for the content of three saponins in D. zingiberensis through screening the spectrum pretreatment method,determining the modeling band and principal component number.Finally,the validation set samples was used to test the prediction accuracy of the three saponin detection models respectively,the content determination methods of deltonin ,zingiberensis newsaponin and dioscin in D. zingiberensis samples met the requirements of quantitative analysis after investigation.The R², RMSEC,RMSEP and PI of the quantitative detection model for deltonin in D. zingiberensis were 0.981 17,0086 3,0.063 8 and 90.5 respectively.The R²,RMSEC,RMSEP and PI of the quantitative detection model of zingiberensis newsaponin were 0.982 64,0.042 0,0.027 4 and 91.1 respectively.The R² of dioscin quantitative detection model was 0.943 64,RMSEC was 0.009 90,RMSEP was 0.005 41,PI was 85.8;Statistical tests showed that there was no significant difference between the predicted and measured values of the three saponins in the three models.The method can be used to determine the contents of deltonin ,zingiberensis newsaponin and dioscin in Dioscorea zingiberensisC.H.Wright relatively quickly and accurately,which provide the basis for rapid quality evaluation of D. zingiberensis.

Key words: HPLC, NIRS, Dioscorea zingiberensis C.H.Wright, PLS, deltonin, zingiberensis newsaponin, dioscin