Abstract: HPLC method was used to monitor the content changes of lobetyolin after being treated with different conditions(temperature,heating time,light,pH,etc.).Result showed the stability of lobetyolin was good under 60 ℃.When the temperature was over 60 ℃,the content of lobetyolin decreased significantly with the increase of temperature and the extension of heating time(P<0.05).After 72 hours of irradiation with 80 Klx,the content of lobetyolin decreased significantly(P<0.05),which would also decrease in the solution with pH>12.In conclusion,the stability of the pure product of lobetyolin was not as good as that in the extract of Codonopsis pilosula,temperature and light had significant effects on the stability of lobetyolin.

Key words: Codonopsis pilosula, lobetyolin, stability, HPLC