Abstract: Sixty-six culturable fungi were isolated from the permafrost in Greater Khingan Mountains by particle isolation method and their natural products were screened for the biological activities.These fungi belonged to 55 taxa using the sequences of Internal Transcribed Spacer (ITS) and of them,the ITS similarities of 8 fungal strains were less than 97%.In those 8 fungal strains,six fungal strains exhibited antimicrobial activities and of them,Exobasidium sp. FSF028 was the strongest one.Podospora sp. FSF042 exhibited the strongest antioxidant activity and Fusarium sp. FSF029 exhibited a significant producing cellulase activity.In addition,this is the also first report that Seltsamia had antioxidant and producing cellulase activities.

Key words: permafrost, culturable fungus, Greater Khingan Mountains, secondary metabolite, antimicrobial activity