Based on data mining and network pharmacology technology,this study summarized the prescription medication rule of ancient doctors in the treatment of AS,and analyzed the drug pairs with the highest frequency of occurrence through network pharmacology,to clarify their pharmacological mechanism,and to provide some reference for clinical medication.The key fields of “bi”,“ridge pain”,“big hunchback” and “rounded hunchback” were searched in the scientific database of TCM,and then the ancient prescriptions were screened,statistically processed,association rules and systematic cluster analysis were conducted.Then,using network pharmacological analysis method,the pharmacological analysis was carried out on the high frequency drug Radix Angelicae Biseratae-Saposhnikoviae Radix (RAB-SR),to clarify its composition,target.action pathway,etc.The results indicated 179 ancient prescriptions are screened out,and 205 Chinese medicines are counted.Twenty-seven Chinese medicines with the use frequency ≥1% are Eucommiae Cortex,Cinnanmomi Cortex,and Saposhnikoviae Radix,etc.,mainly including drugs to tonify deficiency,drugs to promote blood circulation and remove blood stasis,drugs to clear heat,drugs to relieve symptoms and drugs to dispel wind dampness.A total of 35 drug combinations with the occurrence times ≥20 was obtained by association rule mining,among which the occurrence times of RAB-SR was the highest.High-frequency medicine to RAB-SR network pharmacology analysis results show that RAB-SR contains 21 active ingredients,corresponding targets for 237,including Beta-sitosterol and Wogonin etc.They might be RAB-SR key components in the treatment of AS;The core targets of drugs acting on diseases include 17 targets such as IL6,TNF,NOS3,PTGS2 and CXCL8.GO enrichment analysis and KEGG pathway analysis showed that the core targets were involved in multiple processes such as inflammatory response and cell response to lipopolysaccharide,and acted on AS through multiple pathways such as TNF signaling pathway,Toll-like receptor signaling pathway and NF-κB signaling pathway.In the treatment of AS,ancient doctors mainly adopted the TCM characteristic treatment of tonifying deficiency,and simultaneously treating the symptoms and root causes of the disease by relieving the surface and clearing heat,promoting blood circulation and removing blood stasis,removing wind and removing dampness,which provides clinical reference value for further application of TCM in the treatment of AS.Network pharmacology analysis showed that RAB-SR medicine for the Beta-sitosterol and Wogonin,possibly through IL6,TNF,NOS3,signaling pathways in the role of PTGS2,CXCL8 targets such as TNF,Toll-like receptors signaling pathways,NF-κB signaling pathways,such as multiple access,so as to play to the role of the treatment of AS,shows that TCM to treat disease for the characteristics of composition,multiple targets,multiple pathways,also for the subsequent further experiment provides theoretical basis and clinical application.